Nyamplung (Calophyllum inophyllum) seeds known as alternative sources of biofuel, have been revealed to contain coumarins compound that is potentially promising for medicinal sources for diseases therapy. Coumarins are components in seeds that need to be eliminated as a waste when biofuel production process is about to be conducted. This valuable coumarins as sources of drugs have added the values and functions of nyamplung seeds. Coumarins are elements of Phenylpropanoids compound which their derivates are pharmacologically essential for having different physiological activities such as anti-HIV, anti-cancer, anti-inflammation, anti-oxidant, anti-bacterial, anti-coagulant, analgesic and comparative immunemodulation. The aim of this study is to examine variations of total coumarins content of nyamplung seeds from 12 genetic materials collected from nyamplung stands (6 Java, 6 outside Java) throughout 7 different islands in Indonesia. Those stands are important geoghraphic and land races of nyamplung in Indonesia. Analyses for coumarins content was undertaken from fresh seed samples and from extracted nyamplung seed (crude oil) using spektrophotometry method developed by Chang (Yu-ying et al., 2005). Results indicate very high value and variations between nyamplung stands in Indonesia for coumarins content both from seeds and from crude oils. The ranges of coumarins content of seeds from Java and outside Java are 0.1010.383 % and 0.2610.412 %, respectively. Variations were found to be higher when obtained from crude oil, both fresh and one year preserved, of materials from 7 islands in Indonesia, which are 0.3281.109 % and 0.2291.330 %, consecutively. Coumarins content and variations between stands have shown to be higher in crude oil than in seeds.
